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a growing chain. Consequently, once the designated short reaction period had come to a close, each DNA polymerisation reaction was expected to contain a range of incomplete DNA sense strands terminating in a dideoxynucleoside. Where ddATP was used, all the incomplete strands were expected to terminate in ddA, where ddGTP was used all were expected to end in ddG, where ddCTP all were supposed to ended in ddC and with ddTTP strands all terminated in ddT. The DNA sense strand products of all four reactions were then resolved side by side on polyacrylamide gel and the radioactivity visualised by photographic plate, resulting in the famous DNA sequencing ladder, wherein each successive ladder line going upwards in order from the bottom of the gel (shorter strands run faster) was used to identify successive deoxynucleoside residues in the deoxynucleoside sequence of sense strand DNA starting from the primer. Nowadays, sequencing is performed with a single DNA polymerisation reaction containing all four ddNTPs differentially labelled with four different types of uorescent label speci c for each ddNTP (see 4) (Figure 3.13). The complete set of individual DNA sense strand products can now be resolved by capillary electrophoresis (CE, see 7) so that each successive uorescent label resolved and identi ed from the beginning of the elution run (shorter strands run faster) is able to correlate with and identify the deoxynucleoside residue sequence of sense strand DNA starting from the primer. Read lengths are now typically from 400 to 800 deoxynucleoside residues from the primer. how to add barcode in excel 2007 Follow these 7 Steps to Install a Barcode Font in Excel + Word
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