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or no growth in vivo, and failure of endothelial function is a common cause of corneal opacity, thus generating the need for many corneal grafts An important tool in determining the ability of presumptive stem cells to differentiate into corneal endothelium is the presence of easily identi able molecular markers of the HCEC cells Foets et al [1992a, b] showed that human corneal endothelial cells express neural cell adhesion molecules (N-CAM), intercellular adhesion molecule-1 (ICAM-1), and the transferrin receptor CD71 detected by monoclonal antibody OKT9 Cell borderassociated zonula occludens-1 (ZO-1), a tight junction protein, has been extensively used to identify corneal endothelial morphology and integrity [Chen et al, 2001, 2005; Ide et al, 2006; Joyce, 2003; Petroll et al, 1997, 2001; Senoo and Joyce, 2000; Zhu and Joyce, 2004].

Figure 14-3: The Multiline option of the Text Field Property inspector opens up a text area for more user information. When you place a multiline text field on a form, you can specify how text entered into that field should wrap. You indicate this using the Wrap field in the Property inspector. You may select one of the following options: Default: With this option selected, the browser determines the scrolling characteristics of the multiline text field. Browsers and browser versions vary in their default handling of wrapping. Off: With this option selected, text entered into a multiline text field does not wrap when it reaches the right edge of the text area; rather, it keeps scrolling until the user presses Enter (Return). Virtual: This option causes text on the screen to wrap when it reaches the right edge of the text area, but not when the response is submitted to the server; the text is sent as one long string without hard carriage returns. Physical: This option causes text to wrap on the screen and converts the soft returns on the screen to hard returns when the data is submitted to the server.

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Antibodies against some of these proteins, particularly N-CAM, antitransferrin receptor monoclonal antibody OKT9, and tight junction protein ZO-1, are commercially available and thus provide the requisite tools to identify human corneal endothelial cells The most important identi cation for differentiated corneal endothelial cells is function The endothelium has a speci c barrier and pump function for maintaining cornea transparency The pump function of corneas as reconstructed in vitro with transplanted cells or endothelial sheet can be measured with an Ussing chamber [Amano, 2003; Mimura et al, 2004, 2005c; Wigham et al, 2000] The most effective assay for pump function is the observation of corneal transparency in vivo in animals with cultured cells transplanted into the anterior chamber and allowed to settle on the Descemet membrane [Mimura et al, 2005b,c] 1151 Isolation and Culture of Human Corneal Endothelial Cells Protocol 119.

Isolation of Human Corneal Endothelial Cells Reagents and Materials Sterile or aseptically prepared Whole human cornea (see Section 113) CMF-Saline G (see Section 1122) Dispase II, 24 U/mL: dilute to 12 U/mL in DMEM/F-12/GASP for use Trypsin/EDTA (see Section 1124) DMEM/F-12/GASP (see Section 1125) DMEM/F-12/2FB: DMEM:Ham s F-12, 1:1, with 2% FBS CMF/GASP (see Section 1123) Scalpel or single-edged razor blades Curved iris scissors, 11 cm (4-3/8 in) Jeweler s forceps, 10 cm (4 in) Corneal scissors, 19-mm blades, sharp tip Colibri suturing forceps, 01 mm Nonsterile Variable-speed rocking mixer.

You can experience one of Netscape 4 s more spectacularly bizarre rendering effects by combining font-size and adjacent heading tags. What would you expect if you defined style rules for three heading tags in this manner

Tip To display a message only when a user s pointer is over an image or link, use one Set Text of Status Bar action, attached to an onMouseOver event, with your desired status-bar message. Use another Set Text of Status Bar action, attached to an onMouseOut event, that has a null string ( ) as the text.

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Listing 3.

It s a good idea and immediately gratifying to use Dreamweaver s Live Data view early and often when designing with dynamic data. Often, adjustments to table-cell sizes are required in order to get the look you want with the actual data. Click the Show Live Data View button on the toolbar to see your page with the desired information in place.

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