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qualitative sketch of the electric eld and channel for a particular set of voltages In the test circuit, we have gate and drain supply voltages and the source is connected to ground We have already seen that when no gate voltage is applied, both pn junctions are reverse-biased and the transistor cannot conduct current (Figure 930(a)) This is the cutoff region of the n-channel enhancement MOSFET When a gate voltage is applied, an electric eld is generated and a channel is formed when the gate-source voltage, vGS , exceeds a threshold voltage, VT (Figure 930(b)) The threshold voltage is a physical characteristic of a given transistor, and can be treated as a parameter (eg, like the junction voltage in a diode or BJT) Note that the channel is narrower near the drain and wider near the source This is because the electric eld is spatially distributed, and is stronger near the source (which is at zero volts) than near the drain (which is at VDD volts) When the channel has formed, conduction can take place between drain and source Note that, as a result of the nature of the n-type channel, charge carriers are exclusively of the negative type; thus MOSFETs are unipolar devices (as opposed to bipolar transistors) Conduction occurs through electrons injected from the n+ source region into the channel (thus the terminology source, analogous to the emitter in a BJT) These electrons are then swept into the n+ drain (analogous to the collector in a BJT) Thus, the direction of current ow is from drain to source, and we shall refer to this current as the drain current, iD Suppose now that the drain-source voltage is xed at some value (Figure 930(c)) Then, as the gate-source voltage is raised above the threshold voltage (so that a channel is formed), the width of the conduction channel increases because of the increasing strength of the electric eld In this mode of operation, the MOSFET acts as a voltage controlled resistor: as the gate voltage is further increased, the resistance of the channel decreases because the channel width increases This phenomenon, however, can take place only up to a certain gate-source voltage: when the difference between gate-source voltage and threshold voltage, vGS VT , equals the ( xed for the moment) drain-source voltage, vDS , the width of the channel reaches a minimum in the vicinity of the drain The channel width is thus reduced near the drain, because the eld strength is near zero at the drain end of the channel (recall that the eld strength is always at a minimum near the drain) This condition is called pinch-off, and the channel is said to be pinched off Once pinch-off occurs, increasing the gate-source voltage will not cause an appreciable increase in drain current since the channel width is xed at the drain, and the MOSFET behaves much like a constant-current source, with the drain current limited to a saturation value by the pinched-off channel width Figure 931(a) depicts the relationship between drain current and gate-source voltage for xed drain-source voltage of an n-channel enhancement MOSFET The pinch-off condition allows us to divide the operation of the n-channel enhancement MOSFET into two major operating regions: the ohmic or triode region (before pinch-off occurs), and the saturation region (after pinch-off has occurred) In the ohmic region, the MOSFET acts as a voltage-controlled resistor; in the saturation region it acts as a current source If we nally allow both the drain and the gate supply to be varied (Figure 930(d)), we can generate a family of curves These curves, depicted in Figure 931(b), are called the drain characteristic curves They represent the behavior of the MOSFET in terms of drain current versus drain-source voltage curves; each curve in the drain characteristic corresponds to a different value of gate-source.

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mutation results from two of the changes. Uracil pairs with adenine instead of guanine, thus leading to a UA base pair in place of a CG base pair; hypoxanthine (H) pairs with cytosine instead of thymine, the original base paired with adenine. Thus, in this case, an HC base pair replaces an AT base pair. Both of these base pairs (UA and HC) are transition mutations. Xanthine, however, pairs with cytosine just as guanine does. Thus, the replacement of guanine with xanthine does not cause changes in base pairing. Like nitrous acid, heat can also deaminate cytosine to form uracil and thus bring about transitions (CG to TA). Apparently, heat can also bring about transversions by an unknown mechanism.

9

30 Drain Current, mA 25 20 15 10 5 0 0 33333 66667 100000 133333 166667 200000 Gate-source Voltage, V

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These sites where this happens are referred to as AP (apurinic-apyrimidinic) sites. If the AP site is not repaired, any of the four DNA bases could be inserted into the new strand opposite the gap ( g. 12.23). If thymine is placed in the newly formed strand, then the original base pair is restored; insertion of cytosine results in a transition mutation; insertion of either adenine or guanine results in a transversion mutation. Of course, the gap is still there, and it continues to generate new mutations each generation until it is repaired. During DNA replication in E. coli, the polymerase tends to place adenine opposite the gap more frequently than it places other bases.

15 13 Drain Current, A 10 8 5 3 0 3 0 28571 57143 85714 114286 142857 171429 200000 Drain-Source Voltage, V

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Ethyl methane sulfonate (CH3SO3CH2CH3) and ethyl ethane sulfonate (CH3CH2SO3CH2CH3) are agents that cause the removal of purine rings from DNA. The multistep process begins with the ethylation of a purine ring and ends with the hydrolysis of the glycosidic (purine-deoxyribose) bond, causing the loss of the base. The molecules of the acridine dyes, such as pro avin and acridine orange ( g. 12.24), are at. Presumably, they initiate mutation by inserting into the DNA double helix, causing the helix to buckle in the region of insertion, possibly leading to base additions and deletions during DNA replication. Crick and Brenner used acridineinduced mutations to demonstrate both that the genetic

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